Since the cytokine-induced secretion of MANF was only shown in pancreas cells [24] and has never been evaluated in other cells, we aimed to assess here if cytokine-induced MANF secretion is a general phenomenon that occurs in multiple cell-lines, and specifically to assess MANF secretion from tumor cells-lines upon IFN-γ activation, as it may have an immunoregulatory role in the tumor microenvironment. This evidence concerns the gene IFNG and neoplasm.