To assess if MANF secretion upon IFN-γ activation is a general phenomenon, MANF secretion following treatment with 100 ng/mL IFN-γ for 48 hr was tested in additional cell lines, including the murine colon adenocarcinoma MC-38 and human hepatoma cell line HepG2. The gene discussed is MANF; the disease is colon adenocarcinoma.