Based on the expression trend of hub genes in GDM, we found that HSP90AA1 was the predicted target of E2F1, UBE2D3 was the predicted target of HCFC1, EGFR was the predicted target of SRY, PSMC4 was the predicted target of ZFX, DDB1 was the predicted target of RUNX1, STAT3 was the predicted target of SPI1, CCND1 was the predicted target of MYBL2, SMAD3 was the predicted target of SUZ12, FOXO1 was the predicted target of TBX3 and PRKCA was the predicted target of YAP1. The gene discussed is SPI1; the disease is gestational diabetes.