ROC curve analysis was implemented to evaluate the capacity of hub genes to distinguish GDM and non-GDM in E-MTAB-6418, HSP90AA1, EGFR, RPS13, RBX1, PAK1, FYN, ABL1, SMAD3, STAT3 and PRKCA, exhibiting better diagnostic efficiency for GDM and non-GDM, and the combined diagnosis of these ten hub genes was more effective. The gene discussed is RPS13; the disease is gestational diabetes.