To further verify the expression level of hub genes in GDM, RT-PCR was performed to calculate the mRNA levels of the ten hub genes identified in the present study (HSP90AA1, EGFR, RPS13, RBX1, PAK1, FYN, ABL1, SMAD3, STAT3 and PRKCA) in GDM. Here, RBX1 is linked to gestational diabetes.