As the cutoff criterion was set to fold change (FC) >2 or <0.5, three differentially expressed glycopeptides were identified in AFP-negative HCC samples compared to LC samples, including APOH_N253 (H5N4S2), PON1_N253 (H5N4S2), and HPTR/HPT_N126/184 (H5N4S1) (Figure 2D). The gene discussed is AFP; the disease is hepatocellular carcinoma.