Using the empirical cumulative distribution function (ecdf) to calculated the expression percentiles of TSGs in an ESCC-P006 cancer cohort, two patients with somatic/somatic double-hit events showed low expression: one in TP53 (5.32%) and one in PTEN (6.38%) (Supplementary Figure 8) (17). This evidence concerns the gene PTEN and esophageal squamous cell carcinoma.