Using immunofluorescence, we found that undifferentiated HMLE-Snail and HMLE-Twist cells, as well as established mesenchymal breast cancer cells, exhibited a cytoplasmic/membranous staining pattern for the LDL-receptor (LDLR), compared with the weak and mostly diffuse staining of vector-transduced epithelial counterparts (HMLE-vector; Figure 5B). This evidence concerns the gene TWIST1 and breast cancer.