Besides the 4 proteins selected for ELISA validation, the initial Luminex screen also identified urine Eotaxin, GROα, IP-10 and MIP-1β as having the capacity to distinguish BC from controls, with ROC AUC values exceeding 0.70 at p < 0.0001, with specificity values exceeding 0.9. This evidence concerns the gene CXCL1 and breast cancer.