Subsequent western blot analysis of cytoplasmic and membrane protein fractions from LA-treated ischemia-injured tissues revealed significantly increased claudin-4 protein in the membrane fraction when compared to untreated ischemia-injured tissues after 240 minutes of recovery (Fig 4B, Claudin-4/β-actin densitometry ratio of 0.80 ± 0.05 in LA-treated, ischemia-injured tissues vs. 0.43 ± 0.06 in untreated, recovered ischemia-injured tissues, P < 0. The gene discussed is ACTB; the disease is ischemia.