Furthermore, when we set the median MPO-DNA ELISA signal (determined with the modified protocol) of AAA patients (296 RU) and healthy donors of the experimental endotoxemia study at baseline (555 RU) in relation to the calibrator sample generated from isolated human neutrophils (1600 RU for 2 x 106 neutrophils/ml), the recorded values equaled 0.4–0.7 x 106 neutrophils/ml which would correspond to about 10% of a normal neutrophil blood count (2–7.5 x 106/ml) stimulated for NETosis. This evidence concerns the gene MPO and serum lipopolysaccharide activity.