TNF and cryohydrocytosis: Regarding the presence of significant fibrosis and cirrhosis, CHC patients with significant cirrhosis (F4) showed significant (p = 0.001) increase in the levels of AST, ALT, TNF-α, AFP, bilirubin, INR, APRI, Hyp index, HA index, and FN index; a decline in the values of vitamin 25(OH)D (ng/mL); and an increase in the levels of PTH (pg/mL) with reduction in platelet counts compared to CHC patients with mild (F0–F1) and significant fibrosis (F2–F4), respectively (Table 3).