Additionally, according to our observation, 71.4% of the divergence was contributed by pleural fluid samples (cases of pleural effusion and multisystem inflammatory syndrome secondary to bronchopneumonia with/without AKI/ hepatitis/ COPD) and 28.5% (focal dyscognitive seizures and neurobrucellosis) of the divergence was accounted for by CSF samples, while none of the serum ADA showed such a divergence between the two assay methods. The gene discussed is ADA; the disease is chronic obstructive pulmonary disease.