In the receiver operating characteristic (ROC) analysis, only TNF-α and TGF-β reached statistical significance for the discrimination of NAFLD, with area under curve (AUC) values of 0.64 and 0.68, respectively, using the following cutoffs: TNF-α, 25.48 pg/mL and TGF-β, 382.6 pg/mL (Fig. 2). The gene discussed is TNF; the disease is metabolic dysfunction-associated steatotic liver disease.