Of the eleven cell lines that responded to BMP4, eight were significantly inhibited by netrin-1, including the MEF line, the ovarian cancer cell line Ovcar-3, the malignant melanoma cell line A375, the breast cancer cell line MDA-MB-415, the human embryonic kidney cell line HEK293, the glioblastoma cell lines TB101 and TB107, and the colorectal cancer cell line HCT116 (Fig. 2). This evidence concerns the gene NTN1 and colorectal cancer.