While these data only indicate TRP120 can colocalize with Fzd5 in THP-1 cells, we confirmed direct interaction by immunoprecipitating TRP120 from the lysate of infected THP-1 cells harvested at 70 hpi, the end of an infection cycle when ehrlichiae have transitioned to the TRP120-expressing DC, which ensures that sufficient levels of TRP120 are expressed to detect interactions with host proteins. Here, FZD5 is linked to infection.