Conditioned medium obtained from MC38 or B16F10 cells blocked phospho-4E-BP1 activation (figure 5F, G), whereas LEU supplementation in tumor cell conditioned medium restored phospho-4E-BP1 levels (figure 5F, G), although fresh medium with higher concentrations of LEU had minimal effect on 4E-BP1 phosphorylation in CD8+ T cells (Figure S3C). This evidence concerns the gene CD8A and neoplasm.