In conclusion, we conclude that miR-720 may become a potential biomarker for the diagnosis of T2D; the increase of the miR-720 level can inhibit the Rab35 protein expression and then activate the PI3K/Akt/mTOR signal pathway related to insulin signal, thus inhibiting insulin secretion of pancreatic β cells (Figure 6). This evidence concerns the gene INS and type 2 diabetes mellitus.