Moreover, mir-22 inhibits autophagy in SW620 and RKO cells by reducing the expression of autophagy marker LC3-II which increases their sensitivity to 5-FU treatment in vitro and in vivo. Similar to the actions of mir-22 which can counteract the action of autophagy inhibitors by targeting B-cell translocation gene 1 (BTG1),14 recently miR-27a-3p depletion has also been shown to increase apoptosis and reduce proliferation of colon cancer cells by upregulating BTG1 and suppressing ERK/MEK signaling pathway.16 The gene discussed is BTG1; the disease is malignant colon neoplasm.