To explore the role of SPRY4 in CRC, we first screened the expression level of SPRY4 and EZH2 in four human CRC cell lines, SW620, SW480, LOVO and HCT116, and a normal human colorectal epithelial cell line, NCM460, via western blotting and RT-qPCR. This evidence concerns the gene EZH2 and colorectal carcinoma.