Immunohistochemical results (both positive and negative controls were set): SDHB (−) (Fig. 3), and tumor cell S-100(+), SOX10(−), CD34 (+), SMA (partial+), DOG-1 (+), CD117(+), KI-67 (positive in 20% + of the subjects and 40% + of the hotspots) (Additional file 1: e-Fig. 1, Additional file 2: e-Fig. 2, Additional file 3: e-Fig. 3, Additional file 4: e-Fig. 4, Additional file 5: e-Fig. 5, Additional file 6: e-Fig. 6, Additional file 7: e-Fig. 7), We used the Zeiss microscope, the type is Axio Scope. Here, ANO1 is linked to neoplasm.