Moreover, in comparison to two other thyroid cancer cell lines with known constitutively activated AKT (Aydemirli et al. 2019) (being XTC.UC1 cells derived from Hürthle cell cancer and BHP 2-7 cells derived from PTC harboring RET/PTC1), baseline pAKT expression levels in JVE404 were ~94% lower (as shown in Supplementary Figs 5 and 6). The gene discussed is RET; the disease is thyroid cancer.