Hence, we conducted the present study and found that (1) TNC promoted glioma cell proliferation and stemness while suppressing glioma cell apoptosis and chemosensitivity to paclitaxel; (2) TNC enhanced PI3K/AKT signaling, and PI3K/AKT signaling activation reversed the effect of TNC on cell function and chemosensitivity to paclitaxel in glioma cells. This evidence concerns the gene AKT1 and glioma.