TNC mRNA was overexpressed in the human glioma cells, including U87 (P < 0.001), LN-229 (P < 0.001), U251 (P < 0.01) and T98G (P < 0.001) cells, compared to the normal human astrocytes (Fig. 1a), and TNC protein expression was also upregulated in U87, LN-229, U251 and T89G cells compared to normal human astrocytes (Fig. 1b). The gene discussed is TNC; the disease is central nervous system cancer.