A further limitation is that we were not able to evaluate the association between the DLS and several known prognosis factors such as tumor budding, the number of lymph nodes examined, tumor location, obstruction, microsatellite instability, TIL, molecular profile (e.g., BRAF and KRAS), desmoplasia, or histologic subtypes11,30,31,39,40. This evidence concerns the gene KRAS and neoplasm.