Likewise, the other immune checkpoint markers—including B7-H3, B7-H4 (tested in the lung cancer tissue microarray positive control), VISTA, LAG3, ICOS, and TIM3—and immune checkpoint stimulatory markers, such as IDO-1 and OX40, showed similar patterns of cellular expression and distribution between IHC and mIF in tonsil and in lung cancer control tissues18. This evidence concerns the gene TNFRSF4 and lung cancer.