Random forest analyses (Step 5, Fig. 4a, b, and Supplementary Fig. 8) identified SERPINA5 as the second top predictor in the representative phenotype (mean minimum depth = 2.32, p < 0.0001), third top predictor in the extreme phenotype (mean minimum depth = 2.97, p < 0.0001), and as the top predictor in the overall discrimination of AD from control (mean minimum depth = 2.18, p < 0.0001). This evidence concerns the gene SERPINA5 and Alzheimer disease.