Another striking difference in G. mellonella larvae compared to mice and other mammalian infection models is that Francisella virulence did not solely depend on T6SS effectors PdpC and PdpD (Fig. 3A to C; see also Fig. S3 in the supplemental material) (4, 5, 15, 16, 29, 55, 56). This evidence concerns the gene PDP1 and infection.