Western blot analysis revealed that the expression of CST1 and NELL2 was significantly upregulated in esophageal cancer cell lines (Eca109, KYSE150) compared with normal esophageal squamous epithelial cell lines (Het-1A), while ADAMTSL4 and ANGPTL7 was significantly increased in the Het-1A cell line and expressed at significantly low levels in Eca109 and KYSE150 (Figure 9). This evidence concerns the gene ADAMTSL4 and esophageal cancer.