Based on the genetic alterations of LUZP1 in cancer specimens as mentioned above, we analyzed intrinsic cellular features altered in cancer, such as cell migration and invasion in vitro, to see how they might be affected with loss of Luzp1. Using CRISPR/Cas9 gene editing directed to exon 1 of murine Luzp1, we previously generated Shh-LIGHT2 mouse embryonic fibroblasts (Taipale et al., 2000) null for Luzp1 (Luzp1–/– cells), and additionally rescued the same cells by expression of human LUZP1-YFP fusion (+ LUZP1 cells) (Bozal-Basterra et al., 2020). This evidence concerns the gene SHH and cancer.