In addition, the E-MTAB-1980 cohort was used to check the clinicopathologic significance of SLC39A8 expression, as shown in Supplementary Figures 3A–E, the expression of SLC39A8 was decreased in ccRCC patients with distant or lymph node metastasis, and decreased progressively with the increase of T stage and neoplasm histologic grade. This evidence concerns the gene SLC39A8 and metastatic malignant neoplasm in the lymph nodes.