NKX2-5 and colorectal carcinoma: Since high expression of both Nkx2.5 and mutated p53 was observed in poor differentiated CRC cell lines (Figure 2D), and p53 mutants were reported as oncogenic proteins that lead to high malignancy (68, 69), CRC cell lines were allocated into high oncogenic p53 (high mutated p53) and low oncogenic p53 (wild-type and low mutated p53) groups.