NKX2-5 and colorectal carcinoma: Nkx2.5 protein level (P = 0.0052 for Wilcoxon matched-pairs signed-rank test, P = 0.0139 for paired t-test) and mRNA expression (P = 0.0085 for Wilcoxon matched-pairs signed-rank test, P = 0.0264 for paired t-test) were observed to be significantly higher in CRC samples than those in NCM samples (Figures 1B–D).