Finally, there were 10 target genes that had a differential relative mRNA expression (difference in ΔΔCt > 2) comparing patients with early and locally advanced stages of NSCLC were compared (ASPM, BIRC5, TOP2A, TK1, ORC6L, DLGAP5, KIAA0101, PRC1, CEP55, and DTL) (Figure 2). This evidence concerns the gene BIRC5 and non-small cell lung carcinoma.