The percentage of CD4+ gated lymphocytes was shown to be significantly higher in EoE and GERD subjects as compared to HC group (p= 0.018) (Fig.1B), and the percentage of CD4+CD25+ gated lymphocytes was significantly increased in GERD subjects in comparison to HC group (p=0.037) (Fig.1C). This evidence concerns the gene CD4 and gastroesophageal reflux disease.