Single-cell sequencing showed that the transcriptome of pTreg (CD4+CD25+CD127<sup>dim/-</sup> cells isolated from PBMC of colorectal cancer patients) and iTreg (CD4+CD25+CD127<sup>dim/-</sup> cells expanded <i>in vitro</i> according to the above regimen) cells were interlaced. Here, IL7R is linked to colorectal cancer.