ROC analysis revealed the optimal cut-off value for MMP-2 as 1.5 ng/mL, which is associated with a sensitivity of 0.88 or 88% (28 of 32 moyamoya disease patients correctly classified), a specificity of 1.00 or 100% (all 14 controls correctly classified), and an accuracy of 91% (42/46). The gene discussed is MMP2; the disease is Moyamoya disease.