Here, we confirmed the IDH1 in RCC suppressor role, and the hotspot mutations IDH1R132H23-25 will lose its inhibitory effect on RCC, we discussed the wild-type IDH1 underlying mechanisms of RCC inhibition, namely through regulating HIF-1α and HIF-2α to influence the occurrence and development of RCC, and the results in vivo and in vitro strict verification. This evidence concerns the gene HIF1A and renal cell carcinoma.