DDIT3 and psychosocial stress measurement: To investigate whether DIM-induced cell death is associated with ER stress, we firstly performed RNA sequencing analysis of cells treated with DIM or DMSO for 24h to identify specific signaling pathway, the data showed that the levels of CHOP mRNA were prominently increased in DIM treated cells compared to control group (Figure 2A), subsequently, DIM significantly increased expression of CHOP protein in a concentration dependent manner (Figure 2B), indicated that the occurrence of ER stress in DIM induced cells death.