Utilizing the subcutaneous tumor transplantation assay in nude mice and orthotopic lung transplantation models in C57BL/6 mice, we confirmed that inactivation of PI3K/AKT signaling pathway inhibited tumorigenicity and metastasis of lung CSCs, while the inhibition of MAPK/ERK signaling pathway showed no significant changes. The gene discussed is MAPK1; the disease is neoplasm.