Some studies measured the protein expression levels of NF-κB signal related factors, such as p-IKKα/β, IKKα/β, p-IκBα, IκBα, p-p65 and p65, and NF-κB signal transduction downstream proteins iNOS, Cox-2 and TNF-α by western blotting in DSS-induced IBD, the results of the study demonstrated that compared with wild-type mice, the expression levels of these proteins in FGL2-knockout mice were increased 64. This evidence concerns the gene NFKBIA and inflammatory bowel disease.