Fig. 4D illustrates that the expressions of PI3K-p110γ and p-Akt were obviously down-regulated but the expressions of E-Cadherin and Slug were increased in the CM-treated MDA-MB-231 cells of group (C+Mφ) and group (IR+Mφ), especially in the group (IR+Mφ) containing both irradiated cancer cells and macrophages. The gene discussed is AKT1; the disease is cancer.