Other highly-mutated bur rarely-reported genes include TTN, MUC4, CDC27, MUC16, PCLO and UBA52, etc. The most frequently mutated genes in WCH-NonHBV-HCC patients are MUC16 and TP53, and only 4 patients in this group had CTNNB1 mutation. The gene discussed is MUC4; the disease is hepatocellular carcinoma.