In terms of CT26 tumor-bearing mice, the combination of NPPA-PTX NPs with aPD-L1 treatment increased the secretions of TNF-α and IFN-γ by 1.57-fold and by 2.03-fold in tumor tissue, by 2.56-fold and by 2.09-fold in serum, respectively. This evidence concerns the gene TNF and neoplasm.