Especially, the combination of NPPA-PTX NPs with aPD-L1 treatment increased the secretions of TNF-α and IFN-γ by 1.87-fold and by 2.34-fold in tumor tissue, by 3.12-fold and by 2.10-fold in serum on 4T1 tumor-bearing mice, respectively. The gene discussed is IFNG; the disease is neoplasm.