Furthermore, since the overexpression of h-TERT and the oxidative stress increase cancer stem cell subpopulation (CSC)31, and CSC subpopulation is identified by CD13332 and CD44+/CD24− phenotype, it is notable that DHSF-BR16 cells showed an up-regulation of CD133 and a CD44+/CD24−/low mixed phenotype, confirming the stemness profile. Here, PROM1 is linked to cancer.