To assess the potential role of DUSP16 in cancer cells, we transfected a full-length human DUSP16 cDNA expressing plasmid into various types of cancer cells, including NPC HK-1 cells, colorectal cancer DLD-1, and HCT116 cells, gastric cancer Nugc3, and breast cancer MDA-MB-231 cells to generate DUSP16 stably transfected clones. This evidence concerns the gene DUSP16 and cancer.