To obtain a global view of DYRK1A-regulated pathways, we analysed the proteomes and phosphoproteomes of U251 glioblastoma cells infected with doxycycline-inducible DYRK1A shRNA (Fig. 1A) or treated with DYRK1A inhibitors leucettine 41 (L41, IC50 = 40 nM)21 and ALGERNON (ALG, IC50 = 77 nM)22. Here, DYRK1A is linked to glioblastoma.