Therefore, in order to further explore the role of CD38 in the TLR4-related pyroptosis-meditated inflammatory signaling pathway and the potential role of CD38 in liver injury caused by bacterial infection, we injected E. coli into the abdominal cavity of mice (WT, CD38−/−, and CD38−/−TLR4mut) and then observed the pathological changes in liver tissues, expression variation of liver function indicator, cytokines, biomarkers of pyroptosis, and signaling pathways to reveal the effect of CD38 deficiency on bacterial infection-mediated sepsis liver injury. This evidence concerns the gene CD38 and bacterial infectious disease.