In endometrial epithelial cells (Figure 6(a)) and endometrial adenocarcinoma cells (Figure 6(b)), after the overexpression of miR-543, the mRNA expression of vimentin, COL16A1, α-SMA, and Fibronectin decreased, while the mRNA expression of CDH1 increased, while miR-543 inhibitor played the opposite role. The gene discussed is COL16A1; the disease is endometrium adenocarcinoma.