According to the promoter sequence of SNHG3, we designed three different sgRNAs (sg-SNHG3-3, sg-SNHG3-2, as well as sg-SNHG3-1) targeting SNHG3 and evaluated their efficiencies in 5637 and SW780 bladder cancer cells inserted with a sg-SNHG3 or sgRNA negative control (sg-NC). The gene discussed is SNHG3; the disease is urinary bladder cancer.