As shown in Fig. 8A, violin plots of immune cells indicated that B cells naïve, T cells CD8, T cells CD4 memory resting, T cells follicular helper, NK cells activated, Macrophages M1, and dendritic cells resting were higher in other sites group, while NK cells resting and Macrophages M0 were distributed higher in bone metastasis group. The gene discussed is CD4; the disease is bone metastasis.