PDK1 and pulmonary arterial hypertension: To verify our findings on glycolysis in vitro and in vivo, we re-analyzed the RNA-seq data of PAH patients from the public database (No.GSE113439) 39, we found an increased expression of pyruvate dehydrogenase kinase isozyme 1 (PDK1, a key enzyme in glycolysis) in the lungs from 15 PAH patients (Figure 6C).