To confirm that NRF2 drives the malignant progression of HNSCC, we knocked down the NRF2 gene using RNA interference in three HNSCC cell lines (HONE-1, Ca9-22-D1, and OEC-M1; Figure S4) and found that both transient (Figure 2A) and stable (Figure S5A-B) knockdown of NRF2 markedly reduced cell migratory, invasive (Figure 2B), and growth (Figure 2C, Figure S5C) capacity compared to control. Here, NFE2L2 is linked to head and neck squamous cell carcinoma.