On the basis that EVOO intake may contribute to an alleviation of the fatty liver grade in patients treated with a hypocaloric diet18 and can attenuate mild stages of NAFLD in C57BL/6J mice13, we tested whether EVOO-based HFDs may prevent development of IR and more severe stages of NAFLD using Ldlr − / − . Leiden mice. Here, LDLR is linked to Hepatic steatosis.