To sum up, in patients with 3 cm or less HCC, based on CTC > 2/3.2 mL, AFP > 20 ng/mL, DCP > 40mAU/mL, maximum tumor diameter > 20 mm and multiple tumor numbers (≥ 2), we designed a simple clinical scoring system to predict tumor recurrence after RFA,which has a certain application value for preoperative clinical decision-making. The gene discussed is AFP; the disease is hepatocellular carcinoma.